I don't know if anyone's ever done a test to find out the for sure answer.
My opinion is: don't worry about it.
There is lots of advice, going back in time who knows how long, about relaxing gun spring: don't leave magazines loaded, don't leave your bolt action rifle cocked when you store it, etc, etc.
I've been ignoring that advice since the mid 1960's, and I have never seen a fatigued spring yet.
I have a theory that most of the advice about springs taking a 'set' comes from Thee Olden Days. Back then, springs were made by hand, and a gunsmith tempered springs by eye-- when it was red enough, to his eye, he quenched it. If he had a good eye, and the lighting was always the same, it mostly worked. Well, mostly.
Today, springs are made in controlled conditions, and with much more even heat. The result is a much better spring, with the correct temper on the entire spring.
Anyway, that's my opinion, and worth every penny ya paid for it
